Written informed consent was obtained from all study participants. Results DNA Analysis Genomic DNA analysis of the proband revealed that she was heterozygous for a novel missense mutation in the prothrombin gene . The nucleotide and proteins numbering system is dependant on the nomenclature recommended by the Human Genome Variation Society.10 The same mutation was detected in her mother and in three other family members with deep-vein thrombosis but not in an asymptomatic relative. On mismatch PCR-RFLP analysis, the amplicon that was treated with PstI shown a 192-bp band and a 212-bp band .Pharmacists can advise sufferers to ensure that they have their controller and rescue medicines required for the length of their trip, plus an updated asthma action program, and any spacers or asthma-related devices. Patients traveling by plane should pack these materials in their carry-on handbag, Dr. Herman noted. 2.Stay up-to-date on immunizations and avoid infections. Pharmacists can remind asthma individuals to get an annual flu vaccine, as well as take preventive procedures against respiratory infections, like washing their hands frequently.