WE from respective cells expressing Flag-tagged mouse MRTF-A had been subjected to IP/IB analysis with the indicated antibodies . 3d,e). Evaluation of F – and G-actin ratios exposed that the G-actin pools in both cell types were comparable .Phosphorylation of MRTF-A plays a critical part in the subcellular localization of MRTF-A and SRF-dependent transcription. Notably, MRTF-A phosphorylation by ERK raises its affinity for G-actin and promotes the nuclear export of MRTF-A. To examine the phosphorylation condition of MRTF-A by ERK in HaCaT and HAoECs cells, we performed immunoblot evaluation with the phospho-specific MRTF-A antibody.You can view the complete Kaiser Daily Health Policy Survey, search the archives, or sign up for email delivery of in-depth coverage of health policy developments, debates and discussions. The Daily Health Policy Report is published for Kaisernetwork.org, a free assistance of The Henry J. Kaiser Family Foundation. Copyright 2009 Advisory Board Kaiser and Company Family Foundation. All rights reserved.. Additional Zenvia Phase III results in multiple sclerosis declared by AVANIR AVANIR Pharmaceuticals, Inc. During the period of the 12-week study in the MS individual subset, Zenvia 30/10 mg met the principal efficacy endpoint by reducing PBA event prices by an incremental 11.9 percent beyond placebo.